Supplemental Material for Edskes et al., 2018
2018
Supplemental Methods_figures_tables.pdf - Provides a detailed description of the culture conditions,
induction of Hermes transposition,
selection of colonies carrying a transposition, extraction of cellular DNA, PCR
amplification and isolation of the junction points between transposon and
chromosomal insertion site, next-generation sequencing of these sites, and
analysis of the data by visual display and by counting insertions per open
reading frame. Exon Intron Counts.xlsx - Gives the insertions in
every yeast open reading frame, distinguishing exons from introns where
appropriate. Sorted Hits.xlsx - Gives prominent hits sorted by functional
group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons
at different locations in the genome. Count Insertions in ORFs and
Introns.txt - The Python program used for counting insertions. LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in
each of 500 genes for which insertions were recovered more frequently in
[ure-o] than in [URE3] cultures.
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