Isolation and Identification of a Cellulose-Producing Bacterial Strain from the Genus Bacillus
2015
Strain ZF-7 was isolated from defective fruits in this study, and its product was determined as bacterial cellulose in view of the results of cellulose special reaction, FTIR spectroscopy analysis, and cellulose hydrolysis experiments. Based on morphological, physiological, and biochemical characteristics, ZF-7 was identified by 16S rDNA sequences and systematic analysis. The results indicated that 16S rDNA sequence of ZF-7 had 99.5 % similarity to the partial sequence of Bacillus amyloliquefaciens, suggesting that ZF-7 was categorized as a species of B. amyloliquefaciens, and denominated as B. amyloliquefaciens ZF-7. The fermentation performance of ZF-7 under shaking and static flasks was inspected preliminary, and the BC production was 6.6 and 6.2 g/L, respectively.
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