LecRK-VII.1, a Lectin Receptor-Like Kinase, Mediates the Regulation of Salt Stress and Jasmonic Acid Response in Arabidopsis

Lectin receptor-like kinases (LecRKs) are a class of membrane proteins found in higher plants. They play important roles in defense against of pathogens, perception of insect feeding, and stress tolerance. LecRK-VII.1 (AT4G04960) is one member of the LecRK family, which contains a conserved lectin domain and a kinase domain. In this study, the lecrk-VII.1 mutants (lecrk-VII.1-1 and lecrk-VII.1-2) showed increased seed germination rates and primary root length compared to ecotype Columbia-0 (Col-0) when treated with different concentrations of NaCl and methyl jasmonate (MeJA). To further confirm the possible role of LecRK-VII.1 in salt and jasmonic acid (JA) responses, we performed a comparative proteomic analysis of Arabidopsis Col-0 and lecrk-VII.1-1 mutant seedlings. In total, 312 proteins were differentially expressed of which 75 were related to stress responses including salt stress and JA response. Among them, decreased expressions of lipoxygenase (LOX) and annexin were confirmed by RT-PCR analyses. Activity of LOX was accordingly reduced in lecrk-VII.1 mutants. These results provide some valuable data to understand the role of LecRK-VII.1 in the response to salt stress and MeJA. In order to understand the role of LecRK-VII.1 in the response to salt stress and MeJA, we studied the phenotypes and expression patterns between the lecrk-VII.1 mutants and wild type. Comparative proteomic analysis of differentially expressed proteins of the lecrk-VII.1 mutants and wild type in Arabidopsis was performed.