Simultaneous Determination of Prostaglandin E 1 and Prostaglandin E 1 Ethyl Ester in Hairless Mouse Skin Homogenate by High-Performance Liquid Chromatography

A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of prostaglandin and prostaglandin ethyl ester in hairless mouse skin homogenate. The sample treatment procedure involved deproteination and precipitation by acetonitrile. and in supernatant were separated in a reversed-phase C18 column without being interfered by other components present in hairless mouse skin homogenate. 9-Anthracenecarboxylic acid was used as an internal standard. The retention times of , 9-anthracenecarboxylic acid and were, 4.5, 9.5 and 18.0 min, respectively. The assay showed linearity from 1 to for both and . Precision expressed as RSD ranged from 2.3 to 14.1 % for and 1.6 to 11.0% for . Accuracy ranged from 100.5 to 119.6 % for and from 98.0 to 103.7% for . This method was employed successfully to follow the time course of concentrations of and in hairless mouse skin homogenate for stability study.