Experimental study on in vitro lamina propria engineering using oral fibroblast and polyglycolic acids

PURPOSE: This study investigated the feasibility of lamina propria engineering in vitro using expanded oral fibroblast(OFC) and Polyglycolic Acids (PGA). METHODS: OFC were isolated by tissue explant method and expanded in vitro. OFC (20×106) of 3rd passage were collected and then seeded onto PGA unwoven fibers to form a cell-scaffold. The constructs were cultured in DMEM +10% FBS. The cell-scaffold constructs were observed continuously by microscope. Small fragments were harvested at 1 week for electromicroscope, histological and RT-PCR analysis. RESULTS: At the sixth day, a neo-lamina propria was formed. HE and Masson stain revealed the formation of collagen fibers. RT-PCR revealed the new forming collagen was mainly typeⅠcollagen. CONCLUSION: lamina propria tissue is possible to engineer in vitro using oral fibroblast and polyglycolic acids. At this basis, we can construct bi-layer tissue engineering oral mucosa in the further research.