MICROPROPAGATION STUDIES IN COCHLOSPERMUM RELIGIOSUM (LINN.)

In the present investigation, an attempt was made to study the effect of different growth regulators viz., BAP, KIN, IBA, IAA, NAA, and 2, 4-D on in vitro multiplication and callus induction, in an important medicinal plant, Cochlospermum religiosum. Use of apical shoots and nodal explants, and different combination of cytokinins and Auxins, in the basic medium, have resulted in maximum multiple shoot formation. Supplementation of MS basic medium with 1.4 mg/l BAP along with 0.2 mg/l IBA, has resulted in production of maximum 10 shoots and supplementation with 1.0 mg/l KIN, 1.0 mg BAP and 1.2 mg/L2, 4-D was responsible for Callus induction. Cochlospermum contains important medicinal properties, secondary metabolites and gum katira which is important in human welfare. Therefore the present investigation was carried