Multi-PCR (Polymerase Chain Reaction) detection method of H5 subtype avian influenza virus variant strain

The invention relates to a method for multi-PCR (Polymerase Chain Reaction) detection of an H5 subtype avian influenza virus variant strain. In the method, RT (Reverse Transcription)-PCR amplification is carried out on corresponding target genes by using three pairs of primers, and a PCR amplified product is subjected to agarose gel electrophoresis and judged according to an electrophoresis result: if a 300bp type specific band appears, an A type influenza virus strain is judged to be; if 173bp subtype specific band appears, a H5 subtype influenza virus is judged to be; and if 559bp specific band appears, a variant strain is judged to be. The invention provides a novel method for rapid diagnosis of avian influenza and the variant strain and guidance for the selection of vaccines.