Genetic polymorphism of two STR loci D1S1609 and D18S976 in a Chinese population

Either blood samples or buccal swaps were taken from healthy unrelated Han individuals living in Jiangsu, Zhejiang, Anhui province and Shanghai city after obtaining their informed consent. A Chelex method was used for extracting genomic DNA (1). PCR was performed in an Eppendorf Mastercycler gradient system and the total volume is 37.5μL. The PCR products were analyzed by non-denaturing polyacrylamide gel electrophoresis and visualized by silver staining (2). Allele designation was established following the recommendations of the DNA commission of the ISFH (3). Genetic data were analyzed using Powerstats program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou’s method (5) and no deviation from Hardy-Weinberg equilibrium was observed. Table 1 contains the summary of allele frequencies and forensic values for the two STR loci. The complete dataset is available upon request at email: yuzhengao@suda.edu.cn