Chemical luminescence immune analysis reagent box for detecting herpes simplex virus type II IgG antibody

The invention discloses a test kit and a preparation method thereof for detecting herpes simplex virus type II IgG antibody, wherein, alkaline phosphatase is adopted to mark the antibodies, and the chemiluminescence zymolyte liquid includes 1,2-butyl dioxide derivatives. The test kit is composed of solid phase envelope carrier, biotinylated antigen, positive and negative reference substance, enzyme-labeled antibody, the chemiluminescence zymolyte liquid and the condensed washing liquid. The device and method belongs to medical detection field, establishes the method of sensitively and quickly detecting herpes simplex virus type II IgG antibody, has better precision, and is a reliable method for detecting herpes simplex virus type II IgG antibody.