Screening parameters for the extraction of Eucommia rubber (EU-rubber) by microbial solid fermentation.

【Objective】The study aimed to investigate changes of carboxymethyl cellulase(CMC)and pectinase activity and the elimination rate of cellulosic materials caused by microbial solid fermentation(Aspergillus D-5)on Eucommia pericarp under different conditions to screen parameters for the extraction of EU-rubber by microbial solid fermentation.【Method】Aspergillus D-5 was used to investigate the effect of different moisture contents of fermentation substrate,initial pH,dosage of ignition agent and culture temperature on CMC and pectinase activity.【Result】The results showed that the moisture content of fermentation substrate,initial pH,dosage of ignition agent and culture temperature had significant effect on the enzyme activity of D-5strain and the elimination rate of cellulosic materials.The optimal conditions for fermentation by D-5 strain was determined:moisture content of fermentation substrate was 60%,initial pH 6.0,culture temperature 30℃,dosage of ignition agent 1.5%.Under these conditions,the CMC and pectinase activity was the highest after 6days of fermentation and the elimination rate of cellulosic materials was 86.43% after 18 days of fermentation.【Conclusion】Removing cellulosic materials by microbial fermentation material is one of the most important ways to extract EU-rubber.D-5strain in the extraction of EU-rubber has certain application value.