Detection of Rsa I gene polimorphism of low density lipoprotein receptor related protein and its influence on serum lipid level in hemodialysis patients

Objectives To investigate the characteristics of dislipidemia and influence by low density lipo-protein receptor related protein （LRP） exon 3 Rsa I gene polymorphism in hemodialysis patients. Methods Se -rum total cholesterol （TC）, triglyeeride （TG）, low density lipoprotein cholesterol （LDLC）, high density lipoprotein cholesterol ＇（HDLC）, apolipoproteins （Apo A1, B, E）and lipoprotein（a） [ Lp（a） ] were measured, polymerase chain reaction - restriction fragment length polymophism （ PCR - RFLP） was used to detect LRP gene polymorphism in hemodialysis patients. Results The serum levels of TG and ApoB were higher significantly in the hemedialysis patients compared to contrals, and the levels of TC, LDLC, HDLC were lower compared to controls. Patients with cardiovascular disorder had higher TG level compared to patients without the disorder. The data of LRP polymorphism detected by PCR - RFLP showed that the allele frequency and the distribution of the LRP genotype of tetranueleotide reapeat sequence and exon 3 did not differ significantly between controls and hemodialysis patients. There was no statistic difference in serum lipid level between hemodialysis patients with different genotype of LRP exon 3. Conclusions Hemodialysis patients were liable to develop hypertrglyeridemia and hypoeholesteroldemia. TG level might related to the development of cardiovascular disorder in hemedialysis patients. There is different influence of LRP Rsa I gene polymorphism on lipid levels between controls and hemodialysis patients. Key words: Hemodialysis;  Lipoproteins, HDL;  Lipids;  Polymorphism （ Genetics ）