Role of mimic of manganese superoxide dismutase in proliferation and apoptosis of gastric carcinoma BGC-823 cells in vitro and in vivo.

Abstract The aim of this study was to investigate the anti-cancer effect and mechanism of mimic of manganese superoxide dismutase (MnSODm) in gastric carcinoma cells in vitro and in vivo. The MTT was used to measure cytotoxicity of the MnSODm. The appearance of apoptotic BGC-823 cells was detected by flow cytometry analysis. Apoptosis proteins, caspase family and Bcl-2 family were viewed by Western blotting. The phosphoinositide 3 kinase (PI3K)/Akt signaling pathway and the NF-κB pathway were also examined. PI3K inhibitor LY294002 was used to examine the involvement of the PI3K/Akt signaling pathway in this apoptosis-inducing effect. BGC-823 cell xenograft serious combined immunodeficiency disease mice were used for the in vivo study. The result showed that MnSODm could induce growth arrest and apoptosis of BGC-823 cells in vitro and in vivo. Further analysis demonstrated the involvement of activation of caspase cascade in MnSODm induced apoptosis. The expression of anti-apoptotic Bcl-2 was decreased, whereas the expression of pro-apoptotic Bax protein was increased. The main mechanisms on MnSODm-induced apoptosis were related to the inhibition of PI3K expression, which inactivated the phosphorylation of Akt involving the prevention of NF-κB phosphorylation and nuclear translocation. This phenomenon could be inhibited by the PI3K inhibitor LY294002. We demonstrated that the mechanisms of MnSODm inhibited the BGC-823 cell proliferation in vitro primarily are related to the induced apoptosis, which appears to be regulated by the PI3K/Akt pathway involving the prevention of NF-κB nuclear translocation, and then triggering the activation of the caspase cascades. Moreover, the anticancer effect in vivo on BGC-823 cells xenografted mice was also due to MnSODm-mediated apoptosis.