STUDIES ON β-PAROTIN II. PURIFICATION OF β-PAROTIN

Acetone fractionation, acrinol treatment and preparative zone electrophoresis were tried to isolate, β-parotin, and it was clarified that the zone electrophoresis was an excellent procedure for the separation of highly purified β-parotin. And, simultaneously, a by-product, which was considered to be parotin, was separated.The isoelectric point of β-parotin was estimated as pH4.2 by using paper electrophoresis, and that of by-product was roughly estimated as pH5.5 to 5.7 from its solubility curve using a buffer solution.