Protective Effects of Enzyme-assistant Extracts of Sargassum fulvellum against AAPH-induced Oxidative Stress in vitro in Vero Cells

In the present study, Sargassum fulvellum was extracted by enzyme-assistant extraction and the proximate compositions of these extracts were determined. The extraction yields of enzyme-assistant extraction were improved 4.67 to 9.67% compared to water extraction. The free radical scavenging activity of these extracts was evaluated using Electron Spin Resonance (ESR) spectroscopy and the cytotoxicity of these extracts was evaluated by measuring the viability of Vero cells. The Alcalase extract (SFA-2), Celluclast extract (SFC), Kojizyme extract (SFK), and Neutrase extract (SFN) were shown stronger free radical scavenging activity and non-toxicity in Vero cells compared with other extracts. The protective effects of SFA-2, SFC, SFK, and SFN against AAPH-induced oxidative stress in Vero cells were evaluated. SFC showed the strongest protective effect that indicated in scavenging 35.33, 38.75, and 47.69% intracellular reactive oxygen species (ROS) and improving 5.89, 8.36, and 12.13% viability at the concentration of 25, 50, and 100 μg/mL in AAPH-induced Vero cells. Therefore, this study demonstrated that enzyme-assistant extraction is an effective extraction method to extract antioxidant from algae, and SFC possesses strong antioxidant activity may be used as an ideal ingredient in pharmaceutical and cosmeceutical industries.