Antihelmintic effect of sambiloto herb infusa (Andrographis paniculata) to Ascaris suum in vitro.

Budiyanti RT, Murkati, Qadrijati I. 2016. Antihelmintic effect of sambiloto herb infusa (Andrographis paniculata) to Ascaris suum in vitro. Bioteknologi 13: 73- 82. Sambiloto (Andrographis paniculata Nees.) contains of saponin, tannin, and andrographolide that has antihelmintic activities. The aim of this research is knowing the existence of antihelmintic effect from infusa of sambiloto herb at Ascaris suum in vitro. The type of this research is experimental research with “ the post test only group control design” method. Animal trial that was used is Ascaris suum. To get the number of each group, it used quote sampling technique. This research was done in three step that used infusa of sambiloto herb concentracy 20%, 40%, 60%, 80%, 100%, aquadest (negative control), and pyrantel pamoate 0,236%. The antihelmintic effect was determined based on LC50, while the effectiveness of antihelmintic was determined based on LT50 that was compared with LT50 pyrantel pamoate, the drug of choice ascariasis disease. Obtained data was tested statistically by Kolmogorov-Smirnov to show the normality of data. Then, Pearson Test was used to show the correlation between sambiloto herb and the mortality of Ascaris suum. Probit Analysis was used to get LC50 and LT50. Result of Kolmogorov-Smirnov test shows the normality data (p=0,2 or p>0,05). Pearson Test shows that there are significant result (p=0,00 or p<0,05) and has the strong relation (r= 0,999). Probit Analysis shows that the LC50 infusa of sambiloto herb is 61,13% and its LT50 is 6 hours 34 minutes. While the LT50 of pyrantel pamoate is 4 hours 16 minutes. Infusa of Sambiloto herb (Andrographis paniculata, Nees.) has antihelmintic effect with the LC50 is 61,13% although that effectiveness less than pyrantel pamoate.