An improved method for the determination of sulphachloropyrazine in meat and liver of broilers during and after their treatment for coccidiosis

The paper presents results of the HPLC determination of sulphachloropyrazine residues (active component of the drug „Esb3 30%”) in muscle tissue and liver of broiler chickens inoculated with laboratory-grown coccidium in the course and after treatment with this sulphonamide.Extraction of sulphachloropyrazine from samples of broiler muscle tissue and liver was carried out with a mixture of solvents dichloromethane-methanol-acetic acid (90:5:5, v/v/v), followed by extract purification by chromatographic separation on a XAD-2 column and elution of sulphachloropyrazine residues with dichloromethane. The HPLC determination of sulphachloropyrazine residues was accomplished on a Bio Sil C-8 HL 5 ?m column with a mobile phase consisting of 60% aqueous solution of acetonitrile and NH3 (pH=9.5), using a UV detector at 254 nm.The method developed allows quantitative determination of the residues of the anticoccidial agent in broiler tissue samples with a detection limit of 0.02 ?g g–1. Recovery of the method for thi...