Cloning,Expression and Immunogenicity Analysis of Campylobacter jejuni peb1A Gene

Based on the C.jejuni genes in GenBank,the peb1A genes was amplified by PCR from C.jejuni genomic DNA.The PCR fragments were cloned into the pMD18-T vector and sequenced.The sequences were compared with the peb1A genes of different kinds of C.jejuni strains to analyze their homology and diversity degree.The DNA fragment of peb1Ag gene of C.jejuni was optimized and synthesized in accordance with the E.coli 's codon preference,it was inserted to the expression vector pET-28a(+).The recombinant expression plasmid pET-28a(+)-peb1Ag was transformed into E.coli BL21(DE3),and the recombinant protein PEB1 was expressed,purified,and detected by Western blot and the mouse immunologic test.The results showed the recombinant protein PEB1 possessed the good immunogenicity.