Anti-inflammatory evaluation and acute toxicity of three food supplements that contain Moussonia deppeana

Abstract Objective To identify the anti-inflammatory activity through two murine models and in the median Lethal Dose (LD 50 ) of three dietary supplements that contain Moussonia deppeana . Methods The anti-inflammatory activity of three dietary supplements (Cicatrisan/Gastricus ® , Gastinol ® , and Gastrovita ® ) EtOH extracts was evaluated by TPA and by carrageenan murine models; also, median Lethal Dose (LD 50 ) was determined. Verbascoside was quantified by High-Performance Liquid Chromatography. β-sitosterol, stigmasterol and the mixture of ursolic and oleanolic acids were identified in all supplements by TLC; however, none of these dietary supplements contain verbascoside. Results For the TPA model, Cicatrisan/Gastricus ® generated a notable effect with 38.24% inhibition. While in the carrageenan model, it also exhibited noteworthy anti-inflammatory activity of ear edema with 66.39% of paw edema inhibition at 150 mg/kg, followed by Gastinol ® and Gastrovita ® with ≈50% at 300 mg/kg. Finally, LD 50 was >2 g/kg for all supplements, when was administered intragastrically and Body Weight (BW) gain in mice was not altered after 14 days. Conclusions Of the three food supplements containing M. deppeana , only the EtOH extract from Cicatrisan/Gastricus ® formulation (tablets) showed significant anti-inflammatory activity in both experimental models and the LD 50 was >2 g/kg.