Optophysiological Characterisation of Inner Retina Responses with High-Resolution Optical Coherence Tomography
2018
Low coherence laser interferometry has revolutionised quantitative biomedical
imaging of optically transparent structures at cellular resolutions. We report the first
optical recording of neuronal excitation at cellular resolution in the inner retina by
quantifying optically recorded stimulus-evoked responses from the retinal ganglion
cell layer and comparing them with an electrophysiological standard. We imaged
anaesthetised paralysed tree shrews, gated image acquisition, and used numerical
filters to eliminate noise arising from retinal movements during respiratory and
cardiac cycles. We observed increases in contrast variability in the retinal ganglion
cell layer and nerve fibre layer with flash stimuli and gratings. Regions of interest
were subdivided into three-dimensional patches (up to 5-15μm in diameter) based on
response similarity. We hypothesise that these patches correspond to individual
cells, or segments of blood vessels within the inner retina. We observed a close
correlation between the patch optical responses and mean electrical activity of
afferent visual neurons. While our data suggest that optical imaging of retinal activity
is possible with high resolution OCT, the technical challenges are not trivial.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
46
References
4
Citations
NaN
KQI