Effect of selective cycloxygenase-2 inhibitor NS-398 on IL-la and TNF-a expression in HaCaT cells in- duced by ultraviolet rays

Objective 1o study the efiect of selective CC）X-2 inhibitor NS-398 on IL-la and TNF-a expression in HaCaT cells induced by UVA/UVB, and further to explore the mechanism on anti human skin photo-damage. Methods The subcultured HaCaT cells were divided into three groups： simple illuminated group was exposed to UVA/UVB directly, NS-398 interfered group was exposed to UVA/UVB after being treated with NS-398 in different concentrations, and the control group was cultured in normal without any treatment. The expression of IL-la and TNF-a in superna- tant was detected by ELISA kit. Results The level of IL-la and TNF-a expression in supernatant from simple illuminated group was remarkably higher than that in the control group, NS-398 inter- fered group showed much lower level than the simple illuminated one, and the expression of IL-la and TNF-a was dependent on the concentration of NS-398. Conclusions NS-398 can reduce IL-la and TNF-a expression in HaCaT cells induced by ultraviolet rays, suggesting the possibility of anti human skin photo-damage effect. Key words: Cycloxygenase-2;  NS-398;  Interleukin-la;  Tumor necrosis factor-a