Expanding the Reach of Recombineering to Environmental Bacteria.

Broadening the application of recombineering technologies in biotechnologically important bacteria poses significant challenges. Aparicio et al. present a vital breakthrough for efficient single-stranded recombineering by utilizing a thermoinducible system in Pseudomonas putida. This offers a simple genome-editing tool towards creating superior biocatalysts for the synthesis of chemicals and for bioremediation endeavors.