In Vitro Methods for Generating Induced Pluripotent Stem Cells

In this chapter, you will get a brief overview of induced pluripotent stem cells (iPSCs) and the advances in cell reprogramming. The generation of pluripotent cells with higher differentiation potential from somatic cells has opened the door for substantial advances in personalized medicine and regenerative medicine applications. You will also learn the basics of inducing pluripotent stem cells from somatic cells and the different factors affecting the reprogramming efficiency. A step-by-step protocol will guide you to different approaches to generate iPSCs from different types of somatic cells and the advantages and disadvantages of each protocol.