Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)

Dynamic FFOCT allows us to see the sub-cellular motion of biological samples. We are able to follow the evolution in the same plane of biological samples for hours thanks to an autofocus procedure. RPE cells are involved in the integrity of retina and vision. We performed a linear scratch assay in RPE cell culture with a surgical scalpel blade, inducing border cell migration at 20.8 µm/h to close the scratch. We also recorded motility of microvilli, thanks to rapid GPU computing. Quantitative live imaging of RPE cell culture with DFFOCT is useful in development of disease models of retinal degeneration.