Oxyradical accumulation and their detoxification by ascorbic acid and α-tocopherol in soybean seeds during field weathering

The role of ascorbic acid and α-tocopherol in detoxification of oxyradicals were observed in different degree of field weathering in seeds of susceptible and tolerant cultivars of soybean [Glycine max (L.) Merr]. Loss of viability and vigour were associated with accumulation of oxyradicals and their inadequate detoxification by ascorbic acid and α-tocopherol. The result of present study shows that level of both ascorbic acid and α-tocopherol decreases because of their utilization during detoxification. But the amount and availability of these antioxidants is not sufficient to quench all the radicals, hence unable to prevent the seeds from deterioration. The extent of seed deterioration is regulated by the accumulation of oxyradical along with the degree of field weathering and availability of these antioxidants. Field weathering inexorably weakens the antioxidant defense mechanism of seeds and leads to rapid deterioration.