Analysis the Diversity of Bacterial Community from M. itinerans Population Rhizosphere Soil in Hainan Using Miseq Sequencing

Musa itinerans Cheesman is a pioneer in the secondary forest of tropical rainforest, which can easily form a large wild population due to its strong clonal growth. In order to understand the bacterial diversity of soil of M. itinerans, IIllumina Mi Seq high-throughput sequencing was used to determine the V3~V4 variation region of16 S r RNA gene in 8 populations of rhizosphere soil of M. itierans in Hainan, and the structure of bacterial communities in different populations was analyzed. The results showed that 8 rhizosphere soils in M. itinerans produced a total of 14 655 OTUs at similar levels of 97%; the highest OTU yield was found from Xuxx21 at 2 324;the lowest OTU yield was sample Xuxx27 at 1 106. The results of the class analysis showed that 25 phyla, 96 classes, 212 orders, 398 families and 768 genus were detected in 8 samples, of which Proteobacteria and Actinobacteria were the dominant floras. The sample Xuxx25 owed a Chao1 index of 3 036 and a Shannon index of 8.23, with the highest abundance and diversity of bacterial flora. The sample Xuxx26 and sample Xuxx27 shared th e highest similarity of bacterial community structure. Further analysis showed that Clostridium, a key functional component of the cellulose-degrading bacterial genus, was found in all 8 samples, with a high proportion of 0.33% in the sample Xuxx24. This study revealed the diversity of bacteria in the rhizospheric soil of M. itinerans and provided basis for the exploitation of microbial resources from this habitat.