Screening the differentially expressed genes in PD-1/PD-L signal pathways in chronic hepatitis B ( CHB ) patients by gene expression microarray

Objective To screen the gene expression profiles in PD-1/PD-L signal pathways in chronic hepatitis B( CHB )patients by gene expression microarray.Methods The differentially expressed genes in peripheral blood samples of 4 cases with CHB and 2 normal people was determined by Gene expression microarray,and then,in combination with preliminary construction of PD-1/PD-L signal pathways,selecting the consistent transcription factors or regulatory protein.The CD40L、BCL-I0、GATA3 and NFAT protein expression were detected by Western blot.Results 838 differentially expressed genes were detected,150 genes were upregulated and 688 genes were down-regulated.13 genes are belonging to the transcription factors or regulatory protein of PD-1/PD-L signaling pathways that we have constructed.In these 13 genes,6 genes (BCL-10、AP、SRY、MYOD1 、ELK4 、NFAT) are down-regulated and 7 genes( CD 40L、GADS、PDK1、IL-4 、GATA3 、CREB、RARG) are down-regulated.Using the Western blotting method,we found that BCL-10 protein expression was up- regulated in CHB patients,but CD 4 0 L,CATA 3 and NFAT proteins expression were down - regulated,which was not related with viral load carrying capacity.Conclusions CD 40L、BCL-10、GATA3 and NFAT.genes may have an important role in regulating the PD-1/PD-L signaling pathway.Further functional study is still need to be done. Key words: Gene chip;  PD-1/PD-L;  Chronic hepatitis B