Method for preparing hyaluronate oligomer according to digestion method, prepared hyaluronate oligomer and application thereof

The invention discloses a method for preparing a hyaluronate oligomer according to a digestion method, a prepared hyaluronate oligomer and an application thereof. Bacillussp. A50 CGMCC NO.5744 is fermented and cultured, thereby obtaining Bacillussp hyaluronidase for degrading hyaluronic acid or salt solution thereof. The method comprises the following steps of: preparing a solution of the hyaluronic acid or salt solution thereof; performing enzymolysis; inactivating; filtering; depositing; and dewatering and drying. According to the invention, the Bacillussp hyaluronidase generated by the Bacillussp is utilized to degrade the hyaluronic acid or salt thereof; the hyaluronate oligomer is prepared according to the steps of dezymotizing, ethanol precipitation, dewatering and drying; the method is simple in operation, mild in condition, free from damage to product structure and free from environmental pollution; the cost of the hyaluronidase as a fermentation source is low; the method is suitable for large-scale industrial production; the prepared hyaluronate oligomer has the advantages of excellent transdermal absorption property, high purity, zero cytotoxicity, strong oxidation resistance, and the like; and the hyaluronate oligomer can be applied to the fields of cosmetics, food and medicines.