광주지역 유통·판매 소고기에서 분리된 병원성대장균의 항생제 내성 및 유전적 특성조사

This study aims at investigating occurrence of pathogenic Escherichia coli (E. coli) associated with high rate of food poisoning in beef samples collected from markets in Gwangju, and the antimicrobial resistance and genetic characterization of the isolated strains. Pathogenic E. coli was detected in 82 (24.4%) of 335 beef samples, and 102 strains were isolated. Of 102 strains, 66 were identified as enterohemorrhagic E. coli (EHEC) and 11 strains harbored both pathogenic genes of EHEC and EPEC. In antimicrobial susceptibility test, 30 strains were resistant to more than 1 antimicrobial. The most frequent antimicrobial resistance observed in the isolated E. coli was to tetracycline, followed by ampicillin, trimethoprim/sulfamethoxazole, and chloramphenicol. Serotyping of isolated E. coli identified serogroups as O26, O91, O103, O104, O111, O113, O121, O128 and O145. Therefore, we suggest that hygienic management of cooking utensils and sufficient cooking are needed so as not to cause cross-contamination when cooking beef.