Influence of Cold Stress on Transcription of HSP90 in Northeast Wild Boar Fibroblasts

To investigate the spatial and temporal expression profiles of HSP90 gene in fibroblasts of the Northeast Wild boar under cold stress.Real-time fluorescent quantification reverse transcriptase PCR(FQ-RT-PCR) was applied to analyze the expression of HSP90 mRNA in the Northeast Wild boar fibroblasts under 4,15,25 and 32 ℃.The results showed that HSP90 mRNA transcription level didn't significantly increase(P0.05) under cold stress treatments at different temperatures;During rewarming culture,HSP90 mRNA transcription levels increased significantly(P0.05) within 8 h of rewarming incubation following preincubation at 4 or 15 ℃ and the peak showed at the 6th hour.While HSP90 mRNA transcription levels didn't increase significantly in the 25 or 32 ℃ preincubation groups within 8 h of rewarming incubation;HSP90 mRNA transcription levels increased with decreasing of the temperature and duration of the cold treatment time when cells were preincubated at 4 or 15 ℃ for 2,4,6 or 8 h followed by a 4 h rewarming incubation.However,rewarming incubation didn't significantly induce transcription of HSP90 mRNA in cells preincubated at 25 or 32 ℃ for 2,4,6 or 8 h.The result indicated that cold stress induced increase of HSP90 mRNA transcription level in fibroblasts of the Northeast Wild boar,did not occur in the stress period during low temperature treatment,but in the cellular stress period after rewarming.Mild cold stress(25-32 ℃) didn't induce a significant increase at HSP90 mRNA transcript levels,while harsh cold stress(4-15 ℃) induced a significant increase at HSP90 mRNA transcript levels which were directly proportional to the intensity and duration of cold exposure at 4-15 ℃.