Lung inflammation associated with acute necrotizing pancreatitis in dogs and mice

There is evidence that dogs suffering from acute necrotizing pancreatitis (ANP) can develop respiratory disease. However, there are no data describing the lung histopathology in clinical cases of ANP in dogs. Therefore, we hypothesized that lungs of dogs that died due to ANP (n=21) will be inflamed along with recruitment of PIMs, a pro-inflammatory cell normally present in ruminants and equines, compared to lungs of control dogs (n=6). Histologic sections of the pancreas graded independently by two blinded pathologists showed the presence of necrosis and infiltration of inflammatory cells (scores 3-10) whereas control dogs received a score of 0. Lungs from ANP dogs showed no (score of 0/6) to moderate (score 3/6) inflammation, but there was no statistical difference between the lung histological score of the control (median ± SEM: 0.5±0.3) and the ANP (median ± SEM: 1.5±0.2) dogs. However, immunohistochemistry revealed an increase in the numbers of septal macrophages/monocytes in lungs of ANP dogs (median...