Microspectrophotometric determination of human leucocyte DNA content in vitro after irradiation

: Microspectrophotometry was used to determine the DNA content of leucocytes cultured in vitro after irradiation. DNA content values ranges are interpreted as showing the presence of cells with a high ploidy and an increase in the number of cells in G2 stage. These findings are seen as an expression of the arrest of mitosis, without interfering with the duplicative capacity of DNA.