Effect of Yi Shen Jiao Nang on Podocytes in Renal Tissue and Urine of Rats with Diabetic Nephropathy

Objective:To observe the effect of Yi shen jiao nang on the podocytes injuries of rats with diabetic nephropathy.Methods:32 rats were randomly grouped into four groups, as control, model,Yi shen jiao nang treatment and Benarapril treatment;8 rats each group.Two days after models’ establishment, the rats were treated with yi shen jiao nang(2.5 g/kg)/Benarapril(2.5 g/kg), ig one a day, the control or model group was treated with the same amount of water. Blood pressure, urinary protein/24 h, serum creatinine(Scr), blood urea nitrogen(BUN), and endogenous creatinine clearance rate(Ccr) were measured,and optics microscope was adopted to observe the renal pathological changes.The podocytes in renal tissue and urine were investigated by electronic microscope and immunofluorescence respectively.Results:After 24 w, average arterial pressure, urinary protein/24 h, Scr and BUN in model group were significantly higher compared with control group(respectively,P0.05). After treatment with Yi shen jiao nang/ Benarapril, the average arterial pressure, urinary protein/24 h, Scr, BUN were reduced (respectively, P0.05).In control group, there was high level expression of podocalyxin.In the model group,expression of podocalyxin was significantly reduced (respectively,P0.05).After treatment of Yi shen jiao nang/ Benarapril, expression of podocalyxin was obviously increased (respectively,P0.05).There were few podocalyxin positive podocytes in urine of control rats, and podocytes were significantly increased in urine of model rats, compared with control rats. After treatment of Yi shen jiao nang/ Benarapril, podocytes were decreased in urine.Conclusion:Yi shen jiao nang protected diabetic nephropathy rat models from kidney damage probably by reducing the podocytes injuries.