A simple and rapid model for hair-follicle regeneration in the nude mouse.

Summary Background Methods for hair-follicle regeneration are important tools for investigating signalling and cytokines during hair-follicle morphogenesis and cycling. Several animal models for hair reconstitution have been established; however, these models have several shortcomings. Aim To develop a simple and rapid model for hair induction in nude mouse. Methods We designed an improved flap model (IFM) for hair regeneration based on the existing flap assay. Histological sections and scanning electron microscopy were used to evaluate the regenerated hair. The fates of grafted cells were traced by fluorescence. The time required for hair induction was analysed and compared. Results IFM produced a large number of normal hairs, and the time required for hair induction using IFM was 20.67 ± 0.67 days, compared with 29.33 ± 0.67 days for the traditional flap assay. Conclusions The time required for hair regeneration is considerably shortened with IFM. We speculate that this is due to increased blood supply at the transplantation sites.