Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers.

Sequence-related ampliied polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multilorum Lam.). Eighteen SRAP primer combinations generated 334 ampliication bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coeficient ranged from 0.4304 to 0.8529, and coeficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into ive groups partly according to their germplasm resource origins.