A study of different germination media for the 'Šampion' walnut cultivar pollen.

Different culture media were tested in an attempt to improve the knowledge of the most suitable germination media for studying the in vitro pollen germination capacity of 'Sampion', a Serbian walnut cultivar. The research was conducted on agar media, as a four-factorial experiment, with different concentrations of agar (0.6%, 0.8% and 1%), sucrose (10%, 15% and 20%), H3BO3 (0 ppm, 200 ppm and 400 ppm) and CaCl2 (0 and 50 ppm). Pollen germination was maximized (39%) when the germination medium contained 0.8% agar, 15% sucrose, 400 ppm H3BO3 and 50 ppm CaCl2. Large and significant differences in pollen germination were observed in response to changing concentrations of agar, sucrose, boric acid and calcium chloride, and strong interaction was identified between all substances used.