[3] Preparation of tyrosinamide—oligosaccharides as iodinatable glycoconjugates

Publisher Summary This chapter describes a protocol that is an approach to isolate large quantities of reducing N-linked oligosaccharides from glycoproteins utilizing a batch procedure. The oligosaccharides are recovered as a protein-free mixture, converted to oligosaccharide-glycosylamines, and then conjugated with tyrosine. The tyrosinamide oligosaccharides are resolved into single structures on preparative RP-HPLC. Each major oligosaccharide contains a β -glycosylamide linkage between GIcNAc and the carboxyl group of tyrosine with only minor amounts (