Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells

The bifunction fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. The approach in which the antigen was tagged by equal molecular GFP allows using the bi-function fusion protein to establish a new method for immunogical diagnosis.