Studies on the interaction between cytochrome c and cis-PtCl2(NH3)2☆

Abstract The interactions between cytochrome c and cis-PtCl 2 (NH 3 ) 2 under different conditions are reported. It is found that cis-PtCl 2 (NH 3 ) 2 is highly selective to bind to sulfur-containing Met residue and the interaction mode of cis-PtCl 2 (NH 3 ) 2 with cytochrome c is different from that of PtCl 4 2− with cytochrome c. The binding sites of platinum complex in the protein were characterized by analysis of the molar ratio of two metal atoms (Pt and Fe), 1 H NMR spectra, UV-vis spectra, and differential pulse voltammetry.