The Application of Microcolumn Coomb's Test in the Diagnosis of Autoimmune Hemolytic Anemia

Objective To investigate the characteristic of the microcolumn Coomb's test and to observe its diagnosis significance in autoimmune hemolytic anemia(AIHA).Methods Competitive microcolumn gel and a routinely used direct antiglobulin test(DAT) were used to study IgG and C3d in 135 cases of AIHA.Results 19 cases were identified in positive by routine typing.24 cases were identified in positive by microcolumn gel, incuding 13 cases in IgG positive, 4 cases in C3d positive and 7 cases both in IgG and C3d positive.There were statistically difference between the microcolumn gel and routine DAT.Conclusion Microcolumn Coomb's test is a sensitive and objective method for the diagnosis of AIHA.