Dynamic contrast in scanning microscopic OCT
2020
While optical coherence tomography (OCT) provides a resolution down
to 1 µm, it has difficulties in visualizing cellular structures due to a
lack of scattering contrast. By evaluating signal fluctuations, a
significant contrast enhancement was demonstrated using time-domain
full-field OCT (FF-OCT), which makes cellular and subcellular structures
visible. The putative cause of the dynamic OCT signal is the
site-dependent active motion of cellular structures in a sub-micrometer
range, which provides histology-like contrast. Here we demonstrate dynamic
contrast with a scanning frequency-domain OCT (FD-OCT), which we believe
has crucial advantages. Given the inherent sectional imaging geometry,
scanning FD-OCT provides depth-resolved images across tissue layers, a
perspective known from histopathology, much faster and more efficiently
than FF-OCT. Both shorter acquisition times and tomographic
depth-sectioning reduce the sensitivity of dynamic contrast for bulk
tissue motion artifacts and simplify their correction in post-processing.
Dynamic contrast makes microscopic FD-OCT a promising tool for the
histological analysis of unstained tissues.
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
24
References
9
Citations
NaN
KQI