Potentiometric titration of reducing carbohydrates

The work to be described consists of following the changes in oxidation reduction potential as glucose solutions is progressively added to an alkaline solution of Hg(II)-EDTate. Preliminary work appears to show that normal sodium hydroxide provides the best alkaline medium for the purpose. The titrations are carried out at 70–80° C. It was also found that there was no need for rigid exclusion of air. The response of the more important carbohydrates upon titration with this reagent are studied and their reduction values ascertained.