The effects of dietary polyunsaturated fatty acids on prostate cancer: Unbiased proteomic and phosphoproteomic studies

Abstract A line of evidence has suggested that early inference and dietary prevention are beneficial in prostate cancer (PCa) patient care. Fish oil (FO), which contains mostly omega-3 polyunsaturated fatty acid, is one of the most widely studied candidate supplements for PCa prevention; however, the molecular mechanism remains elusive. The goal of this study is to evaluate the regulatory role of FO on PCa through a global unbiased proteomic analysis and a global phosphoproteomic profiling. In the global protein expression study, we found only a few proteins with statistically significant changes, including sequestosome-1 (p62), MSMP, a newly identified proinflammation factor, and a few in the glycolysis pathway. In the global phosphoproteomic study, we confidently identified 828 phosphopeptides from 361 phosphoproteins with FDR