Cytotoxic, anti-HIV–1 and anti-inflammatory activities of lanostanes from fruits of Garcinia speciosa

Abstract Bioassays-guided isolation of the hexanes and ethyl acetate extracts of the fruits of Garcinia speciosa led to the isolation of three new lanostane triterpenes 1 – 3 together with three related known derivatives 4 – 6 . The new structures elucidated on the basis of spectroscopic and X-ray diffraction techniques were identified to be methyl (22 E ,24 E )-9α-hydroxy-3-oxolanosta-22,24-dien-26-oate ( 1 ), (22 E ,24 E )-9α,11 β -dihydroxy-3-oxolanosta-22,24-dien-26-oic acid ( 2 ), and (24 E )-23-acetoxy-3-oxolanosta-9,24-dien-26-oic acid ( 3 ), namely garciosaterpenes D, E and F, respectively. All compounds were evaluated for their cytotoxic and anti-inflammatory activities along with anti-HIV–1 activity. Most of the compounds were found inactive in the cytotoxic assay except for compound 6 which exhibited selective cytotoxicity against a KB cell line. All compounds showed significant inhibitory activities against HIV–1 reverse transcriptase (RT) assay and in the syncytium inhibition assay. Compounds 1 , 5 and 6 displayed promising inhibitory activity in anti-inflammatory process.