Pancreatic cancer protein biomarker detection kit and detection system

2015 
The invention provides a pancreatic cancer protein biomarker detection kit including a CRP monoclonal antibody, an ICAM-1 monoclonal antibody, an OPG monoclonal antibody, a CA19-9 monoclonal antibody, and an enzyme-labelled secondary antibody for forming double antibody sandwich. The invention also provides a pancreatic cancer detection system comprising the detection kit. According to a detection chip and the detection kit, four pancreatic cancer protein biomarkers of CRP, ICAM-1, OPG and CA19-9 obtained through screening by means of 'subtraction method' are subjected to immunization to respectively prepare the specific CRP monoclonal antibody,the specific ICAM-1 monoclonal antibody, the specific OPG monoclonal antibody and the specific CA19-9 monoclonal antibody to be used as the basis, and the enzyme-labelled secondary antibody with double antibody sandwich is combined, and through antigen-antibody specific combination and combination of the enzyme-labelled secondary antibody with double antibody sandwich, biomarking proteins are detected, and higher accuracy and specificity are achieved.
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