Effect of Astragalus Polysaccharide on Expression of Nuclear Factor-κB in Human Cardiac Microvascular Endothelial Cells after Hypoxia and Reoxygenation Injury

Objectives: To observe the expression of nuclear factor-κB(NF-κB) in human cardiac microvascular endothelial cells that were damaged after Hypoxia and Reoxygenation and to explore the mechanism of astragalus polysaccharide(APS) on reducing myocardial ischemia and reperfusion injury.Methods:The ischemia and reperfusion injury model was established by oxygen deprivation and recovery method.Using immunocytochemical stain method to observe the location of NF-κB protein and applying fluorescence quantitative polymerase chain reaction method to detect the expression of NF-κB mRNA.Results: The NF-κB metastasized to cell nuclear after hypoxia/reoxygenated and expression of its mRNA increased obviously compared with the control group(P0.05).APS could inhibit nuclear metastasis of NF-κB and reduce the expression of NF-κB mRNA,and there was significant difference between 50μg/mL of APS group compared with hypoxia/reoxygenated group(P0.05).Conclusion:APS could influence the activation of inflammatory related gene through inhibiting the NF-κB signal transduction pathway,thus alleviating the inflammation reaction of myocardial reperfusion injury.