Analysis of TNFbeta gene microsatellite polymorphism in Graves disease

The work is to study the microsatellite polymorphism of tumor necrosis factor βgene(TNFc)in Graves disease.The TNFc microsatellite polymorphism in the first intron of tumor necrosis factorβgene was amplified by polymerase chain reaction.The allele frequency and genotype frequency of all samples were analyzed by polyacrylamide gel electrophoresis.The results showed that TNFc microsatellite polymorphism included two alleles and three kinds of genotype.The TNFc2 allele frequency in Graves disease group was greater than control group(χ2 = 4.02,P0.05),indicating this allele may have an important role in the pathogenesis of Graves disease.