A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site

Sarah Maxel,Derek Aspacio,Edward King,Linyue Zhang,Ana Paula Acosta,Han Li

A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site

2020

Sarah Maxel
Derek Aspacio
Edward King
Linyue Zhang
Ana Paula Acosta
Han Li

We report an aerobic, growth-based selection platform founded on NADP(H) redox balance restoration in Escherichia coli, and demonstrate its application in high-throughput evolution of an oxygenase....

Keywords:

Hydroxybenzoate
Photochemistry
Oxygenase
Biochemistry
Chemistry
Redox
Escherichia coli
Active site

Correction
Source
Cite
Save
Machine Reading By IdeaReader

References

Citations