HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line

Abstract To screen several peptides that could possibly inhibit the biosynthesis of cholesterol, we selected peptides with high 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) inhibitory activity from silkworm pupae protein hydrolysates (SPPHs). SPPH was obtained by hydrolysing the proteins with neutral proteinase, and filtered to 5 kDa fractions. Fraction 4 (SPP) with the highest HMGCR inhibitory activity was acquired from the ultrafiltrate (