The Chinese traditional medicine ‘Bushen Yinao Pian’ increased the level of ageing-related gene LRPAP-1 expression in the cerebral tissue of accelerated senescence-prone mouse 8/Ta

Abstract The molecular mechanism of the Chinese traditional medicine ‘Bushen Yinao Pian’ (a complex prescription used for clinical anti-ageing in China for over 20 years) is elusive. In this study, the cDNA of low-density lipoprotein related-receptor associated protein-1 ( LRPAP-1 ), an ageing-related gene, which functions as a chaperon or escort protein in the intracellular transport of low-density lipoprotein related-receptor, a transporter of amyloid beta protein (AβP), had been cloned by screening cDNA library based on analyzing the gene expression in cerebral tissue between the test and the control accelerated senescence-prone mouse 8/Ta (SAMP8/Ta). The result shows that this complex prescription increased the expression level of LRPAP-1 . It indicated that the Chinese traditional medicine ‘Bushen Yinao Pian’ plays an important role in anti-ageing by increasing LRPAP-1 expression level.