The tumor suppressor gene 5 in the study of differences in regulatory mechanisms and the molecular level in the process of human gastric cancer

Objective To explore the role of tumor suppresser gene tumor suppressor gene 5 (ST5) in the occurrence of gastric adenocarcinoma. Methods DNA methylation chip and bioinformatic analysis were applied to deep the different genes between diffused gastric adenocarcinoma and normal gastric tissues. The mRNA and protein expression level of ST5 gene were detected by reverse transcription-polymerase chain reaction (RT-PCR) and westen-blot in gastric adenocarcinoma tissues and gastric cancer cell lines. Results The ST5 gene showed significant high methylation in diffused gastric adenocarcinoma; the mRNA expression level of ST5 gene was lower in poorly differentiated SGC-7901 stomach cancer cell lines (fold change=0.61, P<0.01); ST5 gene was demonstrated lower protein expression level in diffused gastric adenocarcinoma tissue and cell lines like BGC-823, MGC-803 and SGC-7901. Conclusion The ST5 gene represented as a tumor suppressor gene, playing an important role in gastric cancerization via regulation of DNA methylation. Key words: Human gastric cancer; DNA methylation chip; Tumor suppressor gene 5